Application

Western blot: 1:1,000 on neonatal rat brain and recombinant human Semaphorin 3A/Fc chimera (95/125 kDa). The antibody reacts with the ~95 kDa protein. The suggested antibody diluent is PBS containing 5% non-fat milk and 0.04% Tween 20.

Optimal working dilutions must be determined by the end user.old>

Anti-Semaphorin 3A Antibody, central region detects level of Semaphorin 3A & has been published & validated for use in WB.

Immunogen

Synthetic peptide from the central region of human semaphorin 3A.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Liquid in PBS containing 50% glycerol, 1 mg/mL BSA and 0.05% sodium azide.

Specificity

It is expected that the antibody will also react with mouse.

Semaphorin 3A. The immunogen sequence has low homology with other semaphorin family members.